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Generation of multicolored, prestained molecular weight markers for gel electrophoresis
Mark M Compton1, Stacey A Lapp, Ron Pedemonte
1Department of Poultry Science, The University of Georgia, Athens, GA 30602-2772, USA. mompton@arches.uga.edu
Abstract:
Molecular weight marker proteins are routinely used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a variety of Remazol-reactive textile dyes. These multicolored proteins provide a set of unambiguous markers for gel electrophoresis. Furthermore, the colored markers can be used in conjunction with Western blotting techniques to provide a visual display of marker proteins on the transfer membrane.