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[Investigation of practical application of fluorescence in situ hybridization (FISH) analysis using microwave
Kazuko Kurosou1, Shigeru Murakami, Mutsuo Jinnai
1Department of Pathology, Dokkyo University School of Medicine, Koshigaya Hospital, Koshigaya 343-8555.
Abstract:
We investigated fluorescence in situ hybridization (FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections of breast fibroadenoma. Higher percentage of cells with 2 signal copies of chromosome 3 centromere could be obtained in the condition of 5 microns thick sections, when we counted cells of more than 4 microns of nuclei in thickness. This method showed about the same results as FISH using cells separated from the same tissues. Percentage of cells with 2 signal copies of chromosome 17 centromere in 14 cases was 80.6 +/- 4.0% (Mean +/- S.D.). This method is expected in the application of the prognosis estimation of the breast cancer.