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[Advanced oxidation protein products in pregnancy]
M Kalousová1, L Fialová, T Zima
1Ustav lékarské biochemie 1. LF UK Praha.
Insights
Advanced oxidation protein products (AOPP) are elevated in pregnant women, indicating increased oxidative stress. These markers do not correlate with prenatal screening results, suggesting hormonal influences.
Area of Science:
- Biochemistry
- Obstetrics
- Oxidative Stress Research
Background:
- Pregnancy and its complications are linked to heightened oxidative stress.
- Advanced oxidation protein products (AOPP) are emerging markers for oxidative stress.
Purpose of the Study:
- To investigate serum AOPP levels in pregnant women during the second trimester.
- To assess the correlation between AOPP and standard prenatal screening parameters.
Main Methods:
- Serum AOPP levels were measured spectrophotometrically in 23 pregnant women.
- Comparison groups included healthy female and male blood donors.
- AOPP were quantified using the Witko-Sarsat method and expressed in chloramin units (mumol/l).
Main Results:
- Pregnant women exhibited significantly higher serum AOPP concentrations compared to female blood donors (P < 0.0001).
- No significant difference in AOPP levels was observed between pregnant women and male blood donors.
- AOPP levels did not correlate with maternal age or prenatal screening markers (HCG, AFP, SP1).
Conclusions:
- Elevated AOPP in pregnant women suggests increased oxidative stress, potentially influenced by hormonal changes.
- The lack of correlation with prenatal screening parameters warrants further investigation.
- AOPP may serve as a sensitive indicator of oxidative stress during pregnancy.
Objective:
Pregnancy and mainly its complications are associated with increased oxidative stress. Advanced oxidation protein products (AOPP) can serve as one of its markers.
Setting:
First Institute of Medical Chemistry and Biochemistry and Institute for Clinical Biochemistry, First Medical Faculty, Charles University; Institute for Care of Mother and Child, Prague.
Methods:
Together with parameters of prenatal screening, AOPP were measured in the serum of 23 pregnant women in the 2nd trimester of pregnancy. A group of healthy blood donors--women and men was used for comparison. AOPP were determined spectrophotometrically according to Witko-Sarsat (absorbance at 340 nm) and are expressed in chloramin units (mumol/l).
Results:
Serum AOPP concentrations in pregnant women are significantly higher in comparison with blood donors--women (85.90 +/- 18.70 mumol/l vs 57.34 +/- 16.31 mumol/l, P < 0.0001) but there is no statistically significant difference between pregnant women and blood donors--men (85.90 +/- 18.70 mumol/l vs 78.60 +/- 44.01 mumol/l). AOPP level does not correlate either with the age of pregnant women or with the parameters of prenatal screening (human chorionic gonadotrophin--HCG, alpha-1-fetoprotein--AFP and trophoblast-specific--beta-1-glycoproteion--SP1).
Conclusion:
AOPP as a marker of oxidative stress is increased in the serum of pregnant women in comparison with women--blood donors but is similar as in men--blood donors which supports the hypothesis of hormonal influence. Nevertheless, AOPP do not correlate with the parameters of prenatal screening (HCG, AFP and SP1).