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Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium
Jeffrey McKinney1, Cecilia Guerrier-Takada, Jorge Galán
1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Journal of Bacteriology
|October 11, 2002
Abstract:
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.