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Updated: May 10, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Published on: October 1, 2010
Somatic cell nuclear transfer
I Wilmut1, N Beaujean, P A de Sousa
1Roslin Institute, Roslin, Midlothian EH25 9PS, UK. ian.wilmut@bbsrc.ac.uk
Nuclear transfer cloning from adult somatic cells shows remarkable developmental plasticity. This process can reverse cell differentiation, enabling a nucleus to direct development to term.
Area of Science:
- Developmental biology
- Cellular reprogramming
- Reproductive technologies
Background:
- Somatic cell nuclear transfer (SCNT) is a technique for creating cloned offspring.
- Adult somatic cells typically have differentiated chromatin structures.
- Oocyte cytoplasm possesses factors capable of reprogramming differentiated nuclei.
Purpose of the Study:
- To demonstrate the developmental plasticity of nuclei from adult somatic cells.
- To investigate the potential for reversing epigenetic modifications governing differentiation.
- To confirm the ability of reprogrammed nuclei to support full development to term.
Main Methods:
- Nuclear transfer of somatic cell nuclei into enucleated oocytes.
- Culture of reconstructed embryos in vitro.
- Transfer of viable embryos into surrogate mothers for gestation.
Main Results:
- Successful development of cloned embryos to term following nuclear transfer.
- Evidence of reversal of somatic cell differentiation markers.
- Demonstration of the oocyte's reprogramming capacity.
Conclusions:
- Cloning via nuclear transfer from adult somatic cells is feasible.
- Oocyte cytoplasm can reprogram differentiated somatic cell nuclei.
- Developmental plasticity is a key feature of nuclear reprogramming.
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