The framework of pathology: good laboratory practice by quantitative and molecular methods

Area of Science:

• Dermatology
• Pathology
• Medical Imaging

Background:

• Traditional histopathological evaluation of dermal collagen is subjective and lacks reproducibility.
• Emerging quantitative image analysis (QIA) methods, like combined confocal laser scan microscopy (CLSM) and Fourier analysis (FA), offer objective and reproducible assessments.
• CLSM/FA by non-pathologists has shown results superior to expert subjective evaluation, meeting Good Laboratory Practice (GLP) criteria.

Discussion:

• Pathology labs face challenges in adopting innovative methods due to a perceived lack of focus on clinical significance, reproducibility, and prognostic value.
• Quantitative methods are reproducible, inexpensive, and offer a good cost-benefit ratio, yet their acceptance is limited.
• A formal study demonstrated successful modernization of a classical pathology laboratory through innovation, leading to high clinician satisfaction.

Key Insights:

• Combined CLSM/FA provides a highly accurate and reproducible method for analyzing dermal collagen orientation.
• Clinician satisfaction with modernized laboratory services reached 96% after implementation of innovative QIA methods.
• Lack of innovative leadership within pathology appears to be a significant barrier to adopting new, effective technologies.

Outlook:

• Non-pathologists are poised to take over laboratory tests historically performed by pathologists, driven by the need for objective and reproducible diagnostics.
• Pathologists must adapt by embracing new technologies and focusing on clinical relevance, reproducibility, and predictive accuracy.
• Future pathology practice should prioritize GLP-compliant, innovative methods that enhance diagnostic value and patient outcomes.