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Improved maintenance of 2,3 DPG and ATP in RBCs stored in a modified additive solution
C F Högman1, F Knutson, H Lööf
1Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden. claes.hogman@klinimm.uu.se
Transfusion
|October 12, 2002
Summary
Erythro-Sol 2 significantly improves red blood cell (RBC) storage by better maintaining 2,3-DPG levels compared to Erythro-Sol 1. This enhanced RBC preservation offers better oxygen-carrying capacity during storage.
Area of Science:
- Biochemistry
- Hematology
- Transfusion Medicine
Background:
- Current red blood cell (RBC) storage methods lead to 2,3-DPG loss, increasing oxygen affinity.
- Previous Erythro-Sol improved 2,3-DPG maintenance with half-strength CPD but not full-strength CPD.
Purpose of the Study:
- To enhance the quality of stored RBCs collected in standard CPD.
- To evaluate a new Erythro-Sol formulation (Erythro-Sol 2) for improved RBC storage.
Main Methods:
- Comparison of Erythro-Sol 2 (150 mL, pH 8.8) with Erythro-Sol 1.
- In vitro assessment of WBC-depleted RBCs stored for 49 days after collection in CPD.
- Utilized automated blood separation (Optipress II).
Main Results:
- Erythro-Sol 2 demonstrated superior maintenance of RBC ATP and 2,3-DPG compared to Erythro-Sol 1.
- ATP levels peaked later in Erythro-Sol 2 (Day 21) vs. Erythro-Sol 1 (Day 14).
- RBC 2,3-DPG increased initially with Erythro-Sol 2, then returned to baseline, while it decreased significantly with Erythro-Sol 1. Hemolysis remained low (<0.17%) in both.
Conclusions:
- Erythro-Sol 2 represents an advancement in additive solutions for RBC storage.
- The formulation effectively preserves key RBC metabolic parameters for extended periods.
