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Updated: Aug 14, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Published on: April 27, 2011
[Preliminary study of development of gene chips for HIV diagnosis]
Ling Li1, Wen-Li Ma, Xiang-Ming Mao
1Institute of Molecular Biology and Key Laboratory of Biochip of PLA, First Military Medical University, Guangzhou 510515, China. liling@fimmu.edu.cn
Objective:
To study the technology for establishing DNA chips for the diagnosis of HIV.
Methods:
HIV 1U26942 DNA fragments were isolated by restriction display-PCR (RD-PCR) and printed onto aminosilane-coated glass slides by Pixsys 5500 arrayer as probes to prepare the gene chips. HIV samples, after labeled with Cy3, were hybridized with the microarray followed by scanning for analysis of hybridization kinetics of the RD fragments.
Results:
The experimental condition for preparing the gene chips was investigated and 12 RD fragments were screened as probes for further study.
Conclusion:
The technique established in this study for preparing DNA chips is specific and applicable.
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