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Screening of tumor necrosis factor-alpha-binding peptides by phage display peptide library
Hai-Ping Guo1, Hai-Bo Luo, Yan-Jun Liu
1Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Objective:
To identify and characterize tumor necrosis factor (TNF)-alpha-binding peptides from c7c phage display peptide library, in an attempt to find short peptides that can be used as antagonist of TNF-alpha.
Methods:
The TNF-alpha-binding peptides were screened from c7c phage display peptide library by using rhTNF-alpha as target protein and identified by sandwich ELISA.
Results:
After 3 rounds of screening, 11 of 23 phage clones were identified as positive clones which can bind to rhTNF-alpha. The amino acid sequence in two of these 11 clones is c-ALWHWWH-c, and that in the others is c-(T/S)WLHWWA-c.
Conclusion:
These phage display peptides are TNFalpha-binding peptides.