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Establishment of dextran sulfate sodium-induced ulcerative colitis model in mice
Qun-Ying Wang1, Cun-Long Chen, Ji-De Wang
1Institute of Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To establish a model of ulcerative colitis in mice.
Methods:
The mice were given 5% dextran sulfate sodium (DSS) solution freely for 7 consecutive days after which distilled water was given in stead for another 10 d, to complete one cycle of whole treatment plan that consisted of 4 such cycles. The symptoms were observed daily. At the end of the first and the fourth cycle respectively, the mice were killed for examining the whole colon under anatomic microscopy and serial tissue sections were prepared for histological observation.
Results:
The symptoms observed in the mice including hematochezia diarrhea and loss of the body weight were similar to those of ulcerative colitis patients. Histological examination revealed infiltration of neutrocytophilia and lymphocythemia, with loss of integrity of the colon mucosa in the gland.
Conclusion:
This mouse model of ulcerative colitis can be easily induced and readily applied in various studies of ulcerative colitis.