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Related Experiment Videos

Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings.

E Bedu1, F Chainier, B Sibille

  • 1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, Centre National de la Recherche Scientifique-Université Claude Bernard Lyon 1, Unité Mixte de Recherches 5123, Villeurbanne, France. elodie.bedu@univ-lyon1.fr

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|October 12, 2002
PubMed
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Growing ducklings adapt to cold by increasing fatty acid synthesis in the liver. This enhanced lipogenesis fuels energy production in thermogenic tissues, crucial for survival in cold environments.

Area of Science:

  • Animal Physiology
  • Metabolic Biochemistry
  • Thermogenesis Research

Background:

  • Birds rely on fatty acid (FA) synthesis and oxidation for thermogenic endurance and cold adaptation.
  • Muscovy ducklings (Cairina moschata) exhibit unique metabolic cold adaptation mechanisms without brown adipose tissue.

Purpose of the Study:

  • To investigate hepatic lipogenesis and FA oxidation capacity in cold-acclimated (CA) ducklings.
  • To determine the impact of cold acclimation on de novo lipogenesis and glucose/acetate metabolism.
  • To explore the role of hepatic lipid synthesis in supporting thermogenesis in growing birds.

Main Methods:

  • Hepatocytes isolated from thermoneutral (TN) and CA ducklings were used to measure FA synthesis rates from glucose and acetate.
  • Enzyme activities of key lipogenic enzymes and glucose oxidation were assessed.

Related Experiment Videos

  • FA oxidation potential was measured in liver and skeletal muscle homogenates.

    • Main Results:

    • CA ducklings demonstrated significantly higher rates of hepatic de novo lipogenesis from both glucose and acetate compared to TN ducklings.
    • Cold acclimation increased hepatocyte glucose oxidation and the activity of key lipogenic enzymes.
    • Enhanced FA oxidation capacity was observed in the liver and skeletal muscle of CA ducklings.
    • Insulin showed a slight stimulatory effect, while glucagon was inhibitory on hepatic lipogenesis in CA ducklings.

    Conclusions:

    • Cold acclimation significantly enhances hepatic lipogenic capacity in growing ducklings, increasing FA synthesis and oxidation.
    • This heightened lipogenic capacity is crucial for metabolic cold adaptation, providing fuel for thermogenic tissues.
    • Muscovy ducks possess a high capacity for lipid synthesis, potentially predisposing them to hepatic steatosis, which is further amplified in cold conditions.