Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Didecyl squarate--a practical amino-reactive cross-linking reagent for neoglycoconjugate synthesis.

A Bergh1, B G Magnusson, J Ohlsson

  • 1Bioorganic Chemistry, Lund University, Sweden.

Glycoconjugate Journal
|October 12, 2002
PubMed
Summary

Researchers synthesized novel hydrophobic squaric decyl ester glycosides for neoglycoconjugate chemistry. These compounds offer easy purification and recovery, enhancing conjugation applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A pilot study of a joint outdoor exercise program for dog owners and dogs.

Scientific reports·2024
Same author

Intersegmental strategies in frontal plane in moderately-skilled riders analyzed in ridden and un-mounted situations.

Human movement science·2019
Same author

Human trophoblast requires galectin-3 for cell migration and invasion.

Scientific reports·2019
Same author

Designing interactions by control of protein-ligand complex conformation: tuning arginine-arene interaction geometry for enhanced electrostatic protein-ligand interactions.

Chemical science·2018
Same author

Vertical head and pelvic movement symmetry at the trot in dogs with induced supporting limb lameness.

Veterinary journal (London, England : 1997)·2017
Same author

Comparing subjective and objective evaluation of show jumping competition and warm-up arena surfaces.

Veterinary journal (London, England : 1997)·2017

Area of Science:

  • Carbohydrate Chemistry
  • Bioconjugation
  • Organic Synthesis

Background:

  • Neoglycoconjugates are crucial for studying carbohydrate-protein interactions.
  • Developing efficient conjugation strategies is essential for neoglycoconjugate applications.
  • Hydrophobic tags can facilitate purification and recovery of modified biomolecules.

Purpose of the Study:

  • To synthesize and characterize novel hydrophobic squaric decyl ester glycosides.
  • To evaluate the utility of these glycosides in neoglycoconjugate chemistry.
  • To demonstrate the advantages of the decyl ester moiety for purification and recovery.

Main Methods:

  • Synthesis of various glycosides (e.g., alpha-D-mannopyranose, lactose, GM3) bearing aminoethyl or aminoethylthioethyl linkers.

Related Experiment Videos

  • Reaction of these glycosides with squaric acid didecyl ester to form hydrophobic derivatives.
  • Conjugation of the synthesized glycosides to amino-functionalized surfaces (microtiter plates, BSA, Sepharose).
  • Purification using silica chromatography and recovery via C18 solid phase extraction.

    • Main Results:

    • Successful synthesis of hydrophobic squaric decyl ester glycosides.
    • Efficient conjugation of these glycosides to various biomolecules and surfaces.
    • Demonstration of traceless hydrophobic tag properties for easy purification.
    • Effective recovery of excess reagents using C18 solid phase extraction.

    Conclusions:

    • Hydrophobic squaric decyl ester glycosides are versatile reagents for neoglycoconjugate chemistry.
    • The decyl ester moiety provides significant advantages in purification and recovery compared to ethyl esters.
    • These findings facilitate the development and application of neoglycoconjugates in various biological studies.