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Updated: Sep 28, 2026

Self-Assembly of Microtubule Tactoids
Published on: June 23, 2022
Microtubule structure at 8 A resolution
Huilin Li1, David J DeRosier, William V Nicholson
1Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA.
Abstract:
We have obtained a 3D reconstruction of intact microtubules, using cryoelectron microscopy and image processing, at a resolution of about 8 A, sufficient to resolve much of the secondary structure. The level of detail in the map allows docking of the tubulin structure previously determined by electron crystallography, with very strong constraints, providing several important insights not previously available through docking tubulin into lower-resolution maps. This work provides an improved picture of the interactions between adjacent protofilaments, which are responsible for microtubule stability, and also suggests that some structural features are different in microtubules from those in the zinc sheets with which the tubulin structure was determined.
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