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Updated: Sep 28, 2026
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
Published on: February 17, 2016
Nogo-A expression in mature oligodendrocytes of rat spinal cord in association with specific molecules
Masanori Taketomi1, Nagatoki Kinoshita, Kazushi Kimura
1Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan. taketomi@anat2.med.kyoto-u.ac.jp
Abstract:
Nogo-A is known as an oligodendrocyte/myelin-associated molecule having an inhibitory effect on neurite outgrowth in the central nervous system. During development, starting from P21 Nogo-A was detected in the cytoplasm of mature oligodendrocytes with compact myelin sheaths in the rat spinal cord. COS7 cells transfected with recNogo-A displayed strong Nogo-A immunoreactivity in their cytoplasm as well as on the mitotic spindle. Nogo-A was not detected in membrane protein fractions from transfected plus biotinylated COS7 cells. Nogo-A was co-immunoprecipitated with alpha-tubulin and myelin basic protein (MBP) from rat brain tissue. These results show that Nogo-A is expressed in association with tubulin and MBP in the mature oligodendrocytes.
