Related Experiment Video
Updated: Aug 18, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
Modulation of age-related biochemical changes and oxidative stress by vitamin C and glutathione supplementation in
1Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt. Sinfac@mum.mans.enn.eg
Abstract:
The present study sought to determine whether supplementation of dietary antioxidant ascorbic acid with glutathione (GSH) could ameliorate the age-related increased oxidative stress and changes in hormonal, lipid and copper (Cu) as well as zinc (Zn) levels in 18-month-old rats. The present study demonstrated that supplementation of vitamin C (30 mg) + GSH 100 mg/kg b.w. significantly reduced the concentration of thiobarbituric acid-reactive substances in liver and testes in old male rats as compared with nonsupplemented ones, indicating lower oxidative stress. In addition, testicular GSH was increased but not hepatic GSH. Also, cholesterol and triglycerides were decreased in the serum of supplemented rats. Furthermore, she serum testosterone level was increased in the same supplemented rats. However, the present results show that the thyroid hormones, T3 and T4, were not influenced. Lastly, the concentration of Cu in serum, liver, brain and testes was increased in supplemented old rats. Zn concentration was also increased in the same organs but not in the liver. According to the present study, the supplementation of antioxidants could play an important role in the modulation of the oxidative damage and changes associated with age.
Related Concept Videos
Mitochondria
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Effect of Aging on Tissues
Aging
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...

