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Related Experiment Videos

Characterization of the Pasteurella multocida hgbA gene encoding a hemoglobin-binding protein.

Montserrat Bosch1, M Elena Garrido, Montserrat Llagostera

  • 1Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.

Infection and Immunity
|October 16, 2002
PubMed
Summary

Pasteurella multocida

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Area of Science:

  • Bacteriology
  • Molecular Biology
  • Pathogenomics

Background:

  • Pasteurella multocida is a significant animal pathogen.
  • Understanding its virulence factors and essential genes is crucial for disease control.
  • Hemoglobin binding is a potential mechanism for iron acquisition in bacteria.

Purpose of the Study:

  • To investigate the function and regulation of the PM0298-PM0299-hgbA transcriptional unit in P. multocida.
  • To determine the role of hgbA in hemoglobin binding and bacterial virulence.
  • To assess the essentiality of PM0298 and PM0299 for bacterial viability.

Main Methods:

  • Reverse transcriptase PCR (RT-PCR) to analyze transcriptional units.
  • Cloning and overexpression in Escherichia coli for protein function studies.

Related Experiment Videos

  • In vitro and in vivo assays for hemoglobin binding and bacterial virulence.
  • Recombinase-based expression technology for in vivo gene regulation studies.
  • Hybridization experiments to assess gene distribution.

    • Main Results:

    • The PM0298-PM0299-hgbA genes form a single transcriptional unit.
    • The hgbA gene product binds hemoglobin in vitro, but hgbA mutants showed no significant virulence defect, suggesting alternative hemoglobin receptors.
    • PM0298 and PM0299 are essential for P. multocida viability.
    • The transcriptional unit is iron-regulated and its expression is induced early during infection in a mouse model.
    • The hgbA gene is prevalent across diverse P. multocida strains.

    Conclusions:

    • The hgbA gene is involved in hemoglobin binding but not essential for P. multocida virulence.
    • PM0298 and PM0299 are essential genes for this bacterial pathogen.
    • The PM0298-PM0299-hgbA operon is tightly regulated by iron and infection.
    • The widespread distribution of hgbA suggests its importance in P. multocida pathogenesis.