Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Base pair mismatch recognition using plasmon resonant particle labels.

Steven J Oldenburg1, Christine C Genick, Keith A Clark

  • 1Seashell Technology, LLC 3252 Holiday Court Suite 227, La Jolla, CA 92037, USA.

Analytical Biochemistry
|October 17, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extinction-to-Absorption Ratio for Sensitive Determination of the Size and Dielectric Function of Gold Nanoparticles.

ACS nano·2020
Same author

The Development and Evaluation of a Statewide Training Center for Home Visitors and Supervisors.

American journal of community psychology·2019
Same author

Bioactivity of nanosilver in <i>Caenorhabditis elegans</i>: Effects of size, coat, and shape.

Toxicology reports·2017
Same author

Biophysics: for HTS hit validation, chemical lead optimization, and beyond.

Expert opinion on drug discovery·2017
Same author

Protein binding modulates the cellular uptake of silver nanoparticles into human cells: implications for in vitro to in vivo extrapolations?

Toxicology letters·2013
Same author

In vitro biodistribution of silver nanoparticles in isolated perfused porcine skin flaps.

Journal of applied toxicology : JAT·2012

Silver plasmon resonant particles (PRPs) offer a 60x more sensitive detection method for DNA hybridization assays. This single particle counting technique provides a robust, low-cost platform for single nucleotide polymorphism analysis.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Molecular Diagnostics

Background:

  • Microarray-based assays are crucial for genetic analysis.
  • Traditional fluorescent labels have limitations in sensitivity.
  • Silver plasmon resonant particles (PRPs) offer unique optical properties.

Purpose of the Study:

  • To evaluate PRPs as reporter labels in DNA hybridization assays.
  • To assess the sensitivity and robustness of PRP-based detection.
  • To screen for a specific polymorphic site in the BRCA1 gene.

Main Methods:

  • Utilized 40-100 nm silver PRPs as reporter labels.
  • Employed a microarray-based DNA hybridization assay.
  • Used dark-field microscopy and CCD imaging for signal detection.

Related Experiment Videos

  • Implemented a software algorithm for counting individual PRPs.

    • Main Results:

    • PRPs were visualized as distinct points of colored light.
    • Detection sensitivity was approximately 60 times greater than fluorescent labels.
    • Single particle counting served as the assay signal.
    • Successfully screened for a known polymorphic site in BRCA1.

    Conclusions:

    • Single particle counting with PRPs is a robust detection method.
    • This approach is generally applicable to various assay platforms.
    • PRP-based systems offer a low-cost, quantitative solution for SNP analysis.