Related Experiment Video
Updated: Aug 18, 2026

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic
R Chakrabarty1, N Viswakarma, S R Bhat
1National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India.
Abstract:
A number of factors that are known to influence genetic transformation were evaluated to optimize Agrobacterium-mediated transformation of hypocotyl explants of cauliflower variety Pusa Snowball K-1. The binary vector p35SGUSINT mobilized into Agrobacterium strain GV2260 was used for transformation and transient GUS expression was used as the basis for identifying the most appropriate conditions for transformation. Explant age, preculture period, bacterial strain and density were found to be critical determinants of transformation efficiency. Using the optimized protocol, the synthetic cryIA(b) gene was mobilized into cauliflower. Molecular analyses of transgenics established the integration and expression of the transgene. Insect bioassays indicated the effectiveness of the transgene against infestation by diamondback moth (Plutella xylostella) larvae
Related Concept Videos
Transgenic Organisms
Bacterial Transformation
Plant Breeding and Biotechnology
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

