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Detection of connective tissue growth factor in human aqueous humor
G-B van Setten1, Timothy D Blalock, Gary Grotendorst
1St. Eriks Eye Clinic, Karolinska Institutet, Stockholm, Sweden. g.van.setten@sankterik.se
Purpose:
Connective tissue growth factor (CTGF) has been shown to be substantially involved in various processes of fibrosis. We investigated if CTGF is present in aqueous humor (AH).
Methods:
Samples from AH were collected from 10 volunteers during cataract surgery. Specific ELISA analysis was performed with goat IGG against human CTGF.
Results:
CTGF was above the detection limit of the assay in 80% of the samples. The concentration of CTGF in the anterior chamber fluid was 1.24 +/- (SD) 0.26 ng/ml.
Conclusion:
CTGF is present in a majority of AH samples, possibly representing a constant component in this fluid. The origin and physiological importance of CTGF is yet unclear. The involvement of CTGF in processes of intraocular fibrosis and wound healing is possible.