Related Experiment Video
Updated: Jul 25, 2026

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Published on: November 20, 2014
Track counting and the blots
1The Nofer Institute of Occupational Medicine, Lódź, Poland. klud@ipm.lodz.pl
Abstract:
Track detectors are very useful devices for alpha particle dosimetry. Alpha particle tracks are registered on the detector's surface. The registered track density is proportional to the dose. When the dose is low the greatest effect on the total error of dose assessment has a Poisson distribution (random error). When the dose is high, the greatest contribution to the total error is the effect of track overlapping (systemic error). This paper describes how to solve the problem of overlapping tracks when a computer aided image analyser is used. The formulae presented in this paper were proven in the international calibration programme run by NRPB and EU.
Related Concept Videos
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

