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Updated: Sep 28, 2026
A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
Voltammetric determination of nitrosocimetidine in simulated gastric juice
M I Walash1, F Belal, F Ibrahim
1Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt. manaleid@mum.mans.eun.eg
Objective:
To develop a voltammetric method for the determination of nitrosocimetidine in simulated gastric juice (SGJ).
Method:
Measurement of the polarographic wave of the nitroso derivative at the dropping mercury electrode in 0.1 m HCl.
Results:
A well-defined diffusion-controlled cathodic wave was obtained at -0.34 V vs. Ag/AgCl electrode. The current-concentration relationship was found to be rectilinear over the range 10-80 microg/mL and 2-50 microg/mL with percentage recovery of 101.24 +/- 0.79 and 100.79 +/- 0.56 for the direct current (DCt) and differential pulse polarography (DPP) modes, respectively, with a minimum detectability (S/N=2) of 0.16 microg/ml (5.6 x 10-7 m) using DPP mode.
Conclusion:
The proposed method was successfully applied to study the nitrosation of cimetidine in SGJ.
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