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Characterization of the SCAN box encoding RAZ1 gene: analysis of cDNA transcripts, expression, and cellular
Tara L Sander1, Jennifer F Morris
1Department of Pathology, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA.
Gene
|October 18, 2002
Summary
The SCAN box protein RAZ1 is widely expressed in human tissues and localizes to the nucleus. This suggests RAZ1 may regulate zinc finger transcription factors, impacting gene expression.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- The SCAN box is a conserved protein association domain found in C(2)H(2) zinc finger proteins.
- SCAN box proteins mediate hetero- and homo-protein interactions.
- RAZ1 (SCAN-related protein associated with MZF1B) is a novel gene identified through yeast two-hybrid screening for MZF1B SCAN box binding.
Purpose of the Study:
- To characterize the RAZ1 gene, including its transcripts, mRNA expression, and protein localization.
- To investigate the potential role of RAZ1 in regulating zinc finger transcription factor function.
Main Methods:
- Analysis of cDNA transcripts and mRNA expression using Northern blot and multiple tissue expression arrays.
- Mapping of RAZ1 transcription start sites via primer extension.
- Determination of RAZ1 protein cellular localization using green fluorescent protein (GFP) fusion and microscopy.
Main Results:
- RAZ1 mRNA is expressed in various human tissues, with highest levels in prostate, testis, thyroid, liver, and kidney.
- The RAZ1 gene produces two transcripts with variant 5'-untranslated regions, both expressing a 28 kDa protein.
- RAZ1 protein localizes to the nucleus in a diffuse pattern, with the SCAN-related domain sufficient for this localization.
Conclusions:
- RAZ1 is a widely expressed nuclear protein.
- RAZ1 may function as a key regulator of zinc finger transcription factor activity.