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Increased dopamine peroxidation in postmortem Parkinsonian brain.

Angela De Iuliis1, Alessandro P Burlina, Roberta Boschetto

  • 1Department of Diagnostics, University of Padua, Viale G. Colombo 3, Italy. angela.deiuliis@unipd.it

Biochimica Et Biophysica Acta
|October 18, 2002
PubMed
Summary

Researchers found increased dopamine (DA) peroxidizing activity in Parkinsonian brains, leading to toxic dopaminochrome formation. This suggests a novel pathway contributing to Parkinson

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Previously identified enzymatic activity in human midbrain catalyzes dopaminochrome formation from dopamine (DA) and hydrogen peroxide.
  • Dopaminochrome is a toxic compound implicated in neurodegenerative processes.

Purpose of the Study:

  • To investigate dopamine (DA) peroxidizing activity in autoptic Parkinsonian brains.
  • To determine if this activity differs between Parkinsonian and normal brain tissues.

Main Methods:

  • Spectrophotometric determination of crude enzymatic activity in extracts of paraffin-embedded brain slices.
  • Analysis of endogenous dopamine (DA) and hydrogen peroxide substrates within brain samples.

Main Results:

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  • Significantly increased DA peroxidizing activity observed in the midbrain of Parkinson's patients compared to controls.
  • DA peroxidizing activity, absent in normal basal ganglia, was detected in all Parkinsonian patients' basal ganglia.

    • Conclusions:

    • A DA peroxidizing pathway is suggested to be present in the human brain.
    • Elevated DA peroxidizing activity in Parkinson's patients generates toxic dopaminochrome, potentially contributing to disease pathogenesis.