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Pitfalls in cefepime titration from human plasma: plasma- and temperature-related drug degradation in vitro
Denis Bugnon1, Eric Giannoni, Paul Majcherczyk
1Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
Antimicrobial Agents and Chemotherapy
|October 18, 2002
Abstract:
While developing a high-pressure liquid chromatography assay for cefepime in plasma, we observed significant drug degradation at 20 and 37 degrees C but not at 4 degrees C. This plasma-related degradation persisted after protein removal. This warrants caution regarding cefepime assays for pharmacokinetic and pharmacodynamic studies of cefepime in vitro and in vivo.