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Cloning of high activity xylanase gene from Bacillus pumilus PJ19
Ainon Hamzah1, Nooraini Abdulrashid
1Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. antara@pkrisc.cc.ukm.my
Abstract:
The xylanase gene from Bacillus pumilus PJ19 amplified by polymerase chain reaction (PCR) was cloned into pCRII vector and transformed into Escherichia coli strain INValphaF'. Starting from an ATG as an initiator codon, an open reading frame coding for 202 amino acids was obtained. The recombinant xylanase sequence showed a 96% homology with the xylanase sequence from B. pumilus IPO strain and had an estimated molecular weight of 22,474. Xylanase activity expressed by E. coli INValphaF' harboring the cloned gene was located primarily in the cytoplasmic fraction.
