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Related Experiment Videos

Is podocyte shape controlled by the dystroglycan complex?

Kenichiro Kojima1, Dontscho Kerjaschki

  • 1Department of Clinical Pathology, University of Vienna, Vienna, Austria.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|October 19, 2002
PubMed
Summary
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Glomeruli contain a specific protein complex that changes in kidney disease. Foot process flattening in glomeruli is linked to the breakdown of this laminin-dystroglycan complex.

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Alpha- and beta-dystroglycan are located at the base of human glomerular foot processes.
  • Podocyte foot process effacement is a hallmark of various proteinuric kidney diseases.

Purpose of the Study:

  • To investigate the composition and role of the laminin-dystroglycan complex in glomerular structure.
  • To explore the relationship between foot process morphology and this complex in kidney disease.

Main Methods:

  • Immunoelectron microscopy was used to detect alpha- and beta-dystroglycan in human glomeruli.
  • Isolated rat kidneys were perfused with protamine sulfate to induce foot process changes.

Main Results:

  • Protamine sulfate rapidly flattened glomerular foot processes in an energy- and actin-dependent manner.

Related Experiment Videos

  • Glomeruli possess substantial amounts of a specific complex involving laminin and dystroglycan.
  • Foot process flattening correlated directly with the dissociation of laminin-dystroglycan complexes.
  • Evidence suggests this complex may be altered in human glomerular diseases.

    • Conclusions:

    • The laminin-dystroglycan complex plays a crucial role in maintaining glomerular foot process structure.
    • Dissociation of this complex is directly associated with foot process flattening.
    • Alterations in the laminin-dystroglycan complex may contribute to the pathogenesis of glomerular diseases.