Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Light damage susceptibility and RPE65 in rats.

Hans Peter Iseli1, A Wenzel, F Hafezi

  • 1Laboratory of Retinal Cell Biology, Department of Ophthalmology, University Hospital Zürich, Switzerland. hanspeter.iseli@aug.usz.ch

Experimental Eye Research
|October 22, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Anti-infectious cross-linking: when and how? : PACK-CXL as treatment option for infectious keratitis].

Die Ophthalmologie·2026
Same author

Durvalumab with carboplatin/paclitaxel and bevacizumab followed by durvalumab and bevacizumab with or without olaparib maintenance in newly diagnosed non-BRCA-mutated advanced ovarian cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

The stability and modulation of if-then rules versus prospective planning in movement selection under dual-tasking conditions.

Scientific reports·2025
Same author

CBCT-based online adaptive radiotherapy of the prostate bed: first clinical experience and comparison to nonadaptive conventional IGRT.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2024
Same author

TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.

Scientific reports·2024
Same author

Visual rehabilitation with mini scleral contact lenses in scarred corneas.

Journal francais d'ophtalmologie·2024

In rats, RPE65 protein levels, not gene sequence variations, influenced light damage susceptibility. Lower RPE65 expression, not sequence changes, may cause reduced levels, suggesting other factors impact rhodopsin regeneration and light damage.

Area of Science:

  • Ophthalmology and Vision Science
  • Genetics
  • Molecular Biology

Background:

  • Retinal Pigment Epithelial 65 (RPE65) protein is crucial for visual pigment regeneration.
  • Sequence variations in RPE65 correlate with protein levels and light damage susceptibility in mice.
  • The role of RPE65 variations in rat vision and light damage is less understood.

Purpose of the Study:

  • To investigate the correlation between RPE65 gene sequence, protein levels, and light damage susceptibility in different rat strains.
  • To determine if RPE65 sequence variations, similar to mice, influence these factors in rats.
  • To explore potential genetic factors contributing to differences in light damage susceptibility in rats.

Main Methods:

  • Examined RPE65 protein and gene sequence in four rat strains.

Related Experiment Videos

  • Quantified RPE65 mRNA levels in albino Wistar and Lewis rats.
  • Assessed rhodopsin regeneration kinetics and light damage susceptibility (LDS) in Wistar and Lewis rats.

    • Main Results:

    • RPE65 protein levels were higher in Lewis and Brown Norway rats than in Wistar and Long Evans rats.
    • Lewis rats exhibited higher Rpe65 mRNA levels than Wistar rats, with no significant Rpe65 sequence variations found.
    • Wistar rats showed greater light damage susceptibility than Lewis rats, despite comparable rhodopsin regeneration.

    Conclusions:

    • Lower RPE65 protein levels in Wistar rats are likely due to decreased gene expression, not sequence variation.
    • RPE65 may not be a rate-limiting factor for rhodopsin regeneration in rats.
    • Unidentified genetic factors, beyond RPE65 levels and rhodopsin regeneration, likely influence light damage susceptibility in rats.