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Irradiation-induced apoptosis of mouse bone marrow stromal cells
Jun Yang1, Zhen-Hua Ding, Cheng-Ji Luo
1Department of Teaching Affairs, First Military Medical University, Guangzhou 510515, China.
Objective:
To study the apoptotic patterns of bone marrow stromal cells (BMSCs) after irradiation.
Methods:
Apoptosis of BMSCs induced by (60)Co gamma irradiation was detected by both flow cytometry and electron microscopy.
Results:
Compared with normal control group, no manifest changes in the apoptosis ratio occurred in BMSCs after 50 Gy irradiation (P>0.05) as demonstrated by flow cytometry. Four hours after 100 Gy irradiation, manifest changes in apoptosis ratio of BMSCs were observed (P<0.01), reaching the peak 20 h later. Similar apoptotic patterns occurred in BMSCs exposed to irradiation of 200 Gy (P>0.05), only the apoptosis ratio was slightly higher.
Conclusion:
Irradiation of proper dose may induce apoptosis in vitro in BMSCs that possess strong resistance to irradiation.