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Updated: Feb 10, 2026

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
Published on: January 31, 2022
[Effect of NADH against liver cell line L02 apoptosis induced by UVB irradiation]
1Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Objective:
To study the molecular mechanism behind the effect of reduced nicotinamide adenine dinucleotide (NADH) against apoptosis of liver cell line L02 induced by ultraviolet B (UVB) exposure.
Methods:
L02 liver cells were exposed to UVB irradiation (200 J/m(2)) followed by a 24-hour culture in the presence or absence of NADH (400 microgram/ml). The degraded fragments of DNA in the cells were observed by agarose electrophoresis, the cell apoptosis rate determined by Annexin/V PI staining and p53, Bax and Bcl-2 proteins expression detected by flow cytometry.
Result:
NADH not only inhibited the apoptosis induced by UVB irradiation, but also up-regulated the expression of Bcl-2 protein and down-regulated expressions of p53 and Bax proteins (P<0.01). Repair of DNA strand damage was observed in L02 cells during the incubation time after irradiation.
Conclusion:
NADH significantly inhibits apoptosis induced by UVB irradiation possibly by the mechanism of up-regulating Bcl-2 expression and down-regulating p53 and Bax expressions.
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