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[Detection of tumor necrosis factor mRNA expression in human cervix and endometrium by in situ hybridization]
1Department of Microbiology, Medical College of Qingdao University, Qingdao 266021, China.
Objective:
To investigate the expression and distributions of tumor necrosis factor (TNF) mRNA in cells derived from human cervix and endometrium with different pathological changes.
Methods:
Non-isotopic in situ hybridization was used to study the locus expression of TNF mRNA in 3 cell lines (SiHa, W12 and NCx) derived from human cervix and 96 paraffin-embedded cervical specimens along with that in 75 specimens of emdometrial tissues.
Results:
TNF mRNA expression was detected in SiHa and W12 cell lines but not in NCx cells. The cervical specimens under examination presented TNF mRNA expression in the cells from the epithelium, interstitial tissues, vascular walls and smooth muscles. The mononuclear cells and smooth muscle cells of endometrial carcinoma tissues, but not those of the normal endometrial tissues, were positive for TNF mRNA expression.
Conclusion:
Epithelial cells derived from condylomatosis and squamous cell carcinoma of the cervix are one type of TNF-producing cells, and the intensity of TNF mRNA expression is related to the degree of uterine hyperplasia. Human papilloma virus infection may enhance TNF mRNA expression in the cervical cells.
