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[Detection of cell apoptosis by MTT assay]
Chun-Qiong Feng1, Wen-Li Ma, Yan-Bing Song
1Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. fengchunqiong@263.net
Objective:
To study the feasibility of using MTT assay to detect cell apoptosis.
Methods:
K562 cells were grown in RPMI 1640 medium supplemented with 10% calf serum and 4 micromol/L arsenic trioxide. Apoptosis was induced in the cultured cells by As2O3, and the cells were detected with optical microscope, DNA gel electrophoresis and MTT staining respectively.
Result:
MTT staining could also accurately detect cell apoptosis, by which the apoptotic cells were easily distinguished from normal cells and dead cells.
Conclusion:
MTT staining is simple, convenient and practical for detecting cell apoptosis.