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[Study on the cloning, expression and the immunogenicity of helicobacter pylori heat shock protein 60 gene]
Yang Bai1, Ya-Li Zhang, Ji-De Wang
1Institute of Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To construct a recombinant strain of bacteria expressing heat shock protein of (Hsp) Helicobacter pylori (Hp) and study the immunogenicity of Hsp60.
Methods:
PCR amplification of Hsp60 DNA was performed before it was inserted into the prokaryotic expression vector pET-22b(+) to transform BL21(DE3) E.coli strain. Hsp60 expressed by the recombinant E.coli was collected and purified for immunogenicity assessment in mice.
Results:
DNA sequence analysis showed identical DNA sequence of Hsp60 thus produced to that published in Genbank. Accounting for a ratio of 27.2% among the total protein production in the bacterium, recombinant Hsp60 protein was recognized by the serum from Hp-infected patients and produced corresponding antibody in Balb/c mice in response to immunization.
Conclusion:
Recombinant Hsp60 protein can be used potentially as a vaccine for controlling and treating Hp infection.
