Related Experiment Video
Updated: Sep 28, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
[Effects of androgen and estrogen on the expressions of basic fibroblast growth factor, transforming growth factor
Wei Tang1, Shao-Bin Zheng, Jin-Hua Zhang
1Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To study effects of androgen and estrogen on the expressions of basic fibroblast growth factor (bFGF) and transforming growth factor beta2 (TGFbeta2) in cultured human prostatic stromal cells.
Methods:
Human prostatic stromal cells obtained from 11 patients with benign prostatic hypertrophy (BPH) were cultured and stimulated with dihydrotestosterone (DHT) and estradiol (E2). The expressions of bFGF and TGFbeta2 mRNA along with smoothelin mRNA were observed with reverse transcriptase-polymerase chain reaction.
Results:
DHT significantly upregulated bFGF expression, and E2 enhanced TGFbeta2 and smoothelin expressions. A positive correlation between expressions of TGFbeta2 and smoothelin was observed.
Conclusion:
DHT can induce bFGF expression and E2 promotes TGFbeta2 expression, and the transformation toward smooth muscle cells induced by E(2) may involve the action of TGFbeta2.
More Related Videos
07:49Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
06:02Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
Published on: June 10, 2016
Related Concept Videos
TGF - β Signaling Pathway
Regulation of Angiogenesis and Blood Supply
Mitogens and the Cell Cycle
Introduction to Fibroblasts
Intracellular Signaling Affects Focal Adhesions
Some...