Related Experiment Video
Updated: Aug 12, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
Published on: December 9, 2016
[CPP32 expression and its significance in multidrug-resistant tumor cells and their parent cells]
Shao-Dong Wang1, Zhen-Shu Zhang, Xing-Quan Zhang
1Institute of Digestive Disease of PLA, Nangfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To study the expression difference of CPP32 in multidrug-resistant (mdr) tumor cells and their parent cells and to understand the possible effect of CPP32 in apoptosis induction in the 2 cell lines.
Methods:
Sequence analysis of CPP32 mRNA extracted form mdr gastric cancer cell line SGC7901/VCR08 and naive cell line SGC7901 was performed by means of reverse transcriptase-polymerase chain reaction (RT-PCR), and the protein expression of CPP32 in the 2 cell lines assayed by way of Western blotting.
Results:
The expressions of CPP32 mRNA was comparable in the 2 cell lines, and sequence analysis found consistent sequence of CPP32 in both cell lines with previous report. No differences was identified in the protein expression, either.
Conclusion:
The apoptosis resistance of mdr cells is not related to the abnormality of CPP32 but the upstream of caspase, the fact of which indicates promising prospect of the research on reversion of mdr cells using CPP32 as target.
Related Concept Videos
Abnormal Proliferation
Treatment Resistant Cancers
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

