Related Experiment Videos
[Detection of apoptosis with gel eletrophoresis combined with computerized gel analysis system]
Qing Zhao1, Jin-Ya Wu, Xing-Xu Huang
1Central Laboratory, First Military Medical University, Guangzhou 510515, China.
Objective:
To explore a new method for detecting cellular apoptosis.
Methods:
Using routine electrophoresis assisted by computerized gel documentation analysis system(GDAS), apoptosis of the macrophages treated with dexamethasone was qualitatively and then quantitatively detected.
Results:
Apoptosis occurred in the macrophages treated with dexamethasone, and DNA fragmentation of the apoptotic macrophages was visualized on 1% agarose gel electrophoresis. The apoptosis rates as determined by computerized GDAS were consistent with the results obtained from flow cytometry that showed typical apoptosis peak.
Conclusion:
Agarose gel electrophoresis assisted by computerized GDAS was relatively simple and less costly in qualitative and quantitative detection of apoptosis with precision.