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Updated: Aug 6, 2026

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
Published on: January 31, 2012
[Identification of genes induced to chondrocytes by nitric oxide]
A I Nikolaev1, V I Dubovaia, D Iu Litvinov
1Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
Abstract:
Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.

