Neuronal degradation in mouse retina after a transient ischemia and protective effect of hypothermia

Temporal profile of neuronal deaths in the mouse retina evoked by a transient retinal ischemia and the protective effect of hypothermia on such deaths were evaluated. A transient ischemic insult was induced in the mouse retina by elevating the intra-ocular pressure. The retina tissue responses after reperfusion were histopathologically detected by monitoring the retinal cell death in the ganglion cell layer and inner nuclear layer, using a sequential TUNEL-staining technique, and by measuring the inner retinal thickness. Elevation of intra-ocular pressure induced a time-related appearance of TUNEL-positive cells in the mouse inner retinas. Peak TUNEL staining occurred 12 h after reperfusion. Lowering mice body temperature to 35 degrees C, 33 degrees C and 29 degrees C during the ischemia period significantly inhibited DNA fragmentation of retinal neurons in a lowering temperature dependent manner. In this experiment, the inner retinal thickness was preserved in 29 degrees C group compared with that in 37 degrees C group. From these results, the 45-min transient ischemia and histopathological examination 12 h later provided a reproducible number of retinal neuronal deaths. Furthermore, hypothermic intervention showed a protective effect to salvage retinal neuronal cells from a transient ischemic insult.