Effect of volatile anesthetics on interhemispheric EEG cross-approximate entropy in the rat

Dissimilarity of the electroencephalogram (EEG) between the two hemispheres was characterized by cross-approximate entropy (C-ApEn), an information statistical parameter applicable to nonlinear, aperiodic signals. EEGs were recorded bipolarly with pairs of epidural electrodes in the left and right frontal cortices. The signals were filtered for 1-100 Hz and digitized at 200 Hz. Inhaled anesthetic concentration was varied between 0.3 and 2.1% with 45-min equilibration periods while the rats were breathing spontaneously. Anesthetics produced concentration-dependent changes in C-ApEn. A greater dynamic range of C-ApEn was obtained by reducing the epoch length from 2 s to 100 ms. At a 0.4% inspired agent concentration halothane caused an increase of C-ApEn, whereas isoflurane did not. When the inspired concentrations of both agents were greater than 0.4%, C-ApEn dose-dependently decreased as agent concentrations increased. Isoflurane depressed C-ApEn more than did halothane at all equivalent minimum alveolar concentration levels, but the two agents became equipotent at 1.5% inspired concentration. C-ApEn fell below the awake baseline at 0.8% anesthetic concentration that also abolished the righting reflex. C-ApEn increased after high-pass filtering (>20 Hz) and decreased after-low pass filtering (<20 Hz) of the digitized EEG; the anesthetic dependence of C-ApEn was diminished by both filters. The results suggest that C-ApEn of bihemispheric EEG is a sensitive, agent-specific correlate of anesthetics' central effect.