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Related Experiment Videos

Tissue engineering skeletal muscle for orthopaedic applications.

Francis C Payumo1, Hyun D Kim, Michael A Sherling

  • 1Department of Pathology, Brown University School of Medicine/The Miriam Hospital, 164 Summit Avenue, Providence, RI 02906, USA.

Clinical Orthopaedics and Related Research
|October 24, 2002
PubMed
Summary

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EClinicalMedicine·2023

Genetically engineered skeletal muscle cells can deliver bone morphogenetic proteins. These engineered cells may serve as a platform for local, long-term delivery of osteoinductive factors for bone regeneration.

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Orthopaedics

Background:

  • Tissue-engineered skeletal muscle analogues (bioartificial muscles) currently lack sufficient force for clinical orthopaedic use.
  • Genetically engineered muscle cells can serve as delivery vehicles for therapeutic proteins in vivo.
  • Bone morphogenetic proteins (BMPs) are key osteoinductive molecules essential for bone formation.

Purpose of the Study:

  • To investigate if skeletal muscle cells engineered to express BMPs can act as a local delivery system for osteoinductive factors.
  • To assess the feasibility of using genetically modified myoblasts for sustained delivery of bone morphogenetic proteins.

Main Methods:

  • Proliferating skeletal myoblasts (C2C12) were transduced with a retrovirus encoding human bone morphogenetic protein-6 (BMP-6).
Keywords:
NASA Discipline MusculoskeletalNon-NASA Center

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  • The resulting C2BMP-6 cells were assessed for constitutive expression and synthesis of bioactive BMP-6.
  • Bioactivity was confirmed by increased alkaline phosphatase activity in coincubated mesenchymal cells.

    • Main Results:

    • C2BMP-6 cells constitutively expressed and synthesized bioactive recombinant human BMP-6.
    • The bioactivity of BMP-6 was retained within the cell layer, not secreted into the medium.
    • Mesenchymal cells coincubated with C2BMP-6 cells showed increased alkaline phosphatase activity, confirming BMP-6 bioactivity.

    Conclusions:

    • Genetically engineered skeletal muscle cells can express and retain bioactive bone morphogenetic proteins.
    • Skeletal muscle cells engineered with BMP-6 show potential as a localized platform for long-term delivery of osteoinductive factors.
    • This approach may offer a novel strategy for enhancing bone regeneration in orthopaedic applications.