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Acyl-CoA binding protein is an essential protein in mammalian cell lines
Nils J Faergeman1, Jens Knudsen
1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. nils.f@bmb.sdu.dk
The Biochemical Journal
|October 25, 2002
Abstract:
In the present work, small interference RNA was used to knock-down acyl-CoA binding protein (ACBP) in HeLa, HepG2 and Chang cells. Transfection with ACBP-specific siRNA stopped growth, detached cells from the growth surface and blocked thymidine and acetate incorporation. The results show that depletion of ACBP in mammalian cells results in lethality, suggesting that ACBP is an essential protein.