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FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: May 2, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 7, 2018

Simultaneous fluorescence immunophenotyping and FISH on tumor cells

Yanming Zhang¹, Brigitte Schlegelberger

¹Department of Medicine, Section of Hematology/Oncology, University of Chicago, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 26, 2002

Summary

No abstract available in PubMed .

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