Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sonication induced sheet formation at the air-water interface.

K S Satheeshkumar1, R Jayakumar

  • 1Bio Organic Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India.

Chemical Communications (Cambridge, England)
|October 26, 2002
PubMed
Summary

A prion peptide (AGAAAA-GAVVGGLGG) forms stable beta-sheet structures in water and at the air-water interface. Sonication further stabilizes this structure, suggesting insights into prion protein folding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic effect of silver nanoparticles and titanium nanotubes loaded chitosan hydrogels for biomedical applications.

International journal of biological macromolecules·2025
Same author

Injectable vancomycin loaded hyaluronic acid-chitosan hydrogel for the treatment of Staphylococcus aureus septic arthritis.

Carbohydrate research·2025
Same author

Electrospun nanofibers of collagen and chitosan for tissue engineering and drug delivery applications: A review.

International journal of biological macromolecules·2025
Same author

Gingival Mesenchymal Stem Cells: A Periodontal Regenerative Substitute.

Tissue engineering and regenerative medicine·2024
Same author

Biomimetic kartogenin containing κ-carrageenan hydrogel for nucleus pulposus regeneration.

International journal of biological macromolecules·2024
Same author

Cough remedy leading to chronic cough: An unusual airway foreign body-Clove.

Respirology case reports·2023

Area of Science:

  • Biochemistry
  • Structural Biology
  • Prion Science

Background:

  • Prion diseases are linked to misfolded prion proteins.
  • The prion peptide PrP (106-127) is implicated in disease pathogenesis.
  • Understanding peptide structure is crucial for disease mechanism research.

Purpose of the Study:

  • To investigate the structural conformations of a specific prion peptide (AGAAAA-GAVVGGLGG).
  • To determine how environmental conditions (aqueous dissolution, air-water interface, sonication) affect peptide structure.
  • To explore the formation of beta-sheet structures relevant to prion aggregation.

Main Methods:

  • Aqueous dissolution of the pentadecapeptide AGAAAA-GAVVGGLGG.
  • Compression at the air-water interface to form a monolayer.

Related Experiment Videos

  • Sonication to induce structural changes.

    • Main Results:

    • The peptide adopts a mixture of random and sheet conformations upon dissolution.
    • Compression at the air-water interface yields a stable monolayer with high beta-sheet content.
    • Sonication leads to the formation of a kinetically stabilized beta-sheet structure.

    Conclusions:

    • The prion peptide exhibits conformational flexibility in aqueous solution.
    • The air-water interface and sonication promote the formation of stable beta-sheet structures.
    • These findings provide insights into the structural basis of prion protein misfolding and aggregation.