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Updated: Aug 14, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
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Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

Single-step isolation of N-linked glycans from deglycosylation reaction mixtures

R M Taylor1, T R Foubert, J B Burritt

  • 1Montana State University, Department of Microbiology, Bozeman 59717, USA. rosst@montana.edu

Biotechniques
|October 26, 2002
PubMed
Abstract

No abstract available in PubMed .

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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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