Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Doc-mediated cell killing in Shigella flexneri using a C1/LacI controlled expression system.

David A Schofield1, Caroline Westwater, Joseph W Dolan

  • 1Department of Microbiology and Immunology, BSB 201, Medical University of South Carolina, 173 Ashley Avenue, Charleston 29403, USA. schofida@musc.edu

FEMS Microbiology Letters
|October 26, 2002
PubMed
Summary

Researchers developed a dual-regulated promoter system for Shigella flexneri, achieving high induction ratios. This system enabled the demonstration of bacteriophage P1

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil Extracellular Trap Formation and Complement Activation Pathways Dominate Microbiota-dependent disease bias in lupus-prone female NZM2328 mice.

bioRxiv : the preprint server for biology·2026
Same author

Human Gut Commensal Bacteroides fragilis Suppresses Mucin Production and Alters Microbiota Composition Resulting in Accelerated Type 1 Diabetes in Mice.

Immunology·2025
Same author

Prevalence of posttraumatic stress disorder and major depression in Ohio, Pennsylvania, and West Virginia 9 months after the East Palestine train derailment.

Journal of traumatic stress·2025
Same author

Gut colonization by <i>Bacteroides fragilis</i> at juvenile age alters microbiota composition and accelerates type 1 diabetes progression in non-obese diabetic mice.

bioRxiv : the preprint server for biology·2025
Same author

Posttraumatic Stress Disorder Among Adults in Communities With Mass Violence Incidents.

JAMA network open·2024
Same author

Who tests for lead and why? A 10-year analysis of blood lead screening, follow-up and CNS outcomes in a statewide US healthcare system.

Occupational and environmental medicine·2024

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Shigella flexneri is a Gram-negative bacterium causing bacillary dysentery.
  • Developing precise genetic control systems is crucial for studying bacterial pathogens.
  • Existing promoter systems may lack the stringency required for certain applications.

Purpose of the Study:

  • To develop a highly stringent and dually regulated promoter system for Shigella flexneri.
  • To evaluate the utility of this system by demonstrating bacteriophage P1's Doc-mediated killing.
  • To establish a novel genetic tool for research in S. flexneri.

Main Methods:

  • Constructed a dual promoter system regulated by bacteriophage P1's temperature-sensitive C1 repressor and LacI.
  • Achieved transcriptional control via the LacI repressor.

Related Experiment Videos

  • Assessed promoter induction/repression ratios in S. flexneri.
  • Demonstrated the bactericidal effect of bacteriophage P1's Doc protein.

    • Main Results:

    • Achieved up to 3700-fold induction/repression ratios in S. flexneri.
    • Confirmed the bactericidal activity of the Doc protein in this species.
    • Established a functional and highly stringent inducible promoter system.

    Conclusions:

    • A novel, dually regulated, and highly stringent promoter system was successfully developed for Shigella flexneri.
    • This system enables precise control over gene expression in S. flexneri.
    • The study demonstrates the first report of Doc-mediated killing in S. flexneri, validating the promoter system's utility.