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Related Experiment Video

Updated: Jul 17, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

[Nitric oxide storage in the cardiovascular system].

E B Manukhina¹, B V Smirin, I Iu Malyshev

¹Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, ul. Baltiiskaya 8, Moscow, 125315 Russia.

Izvestiia Akademii Nauk. Seriia Biologicheskaia

|October 29, 2002

Summary

Nitric oxide (NO) is stored in the body by S-nitrosothiols and dinitrosyl iron complexes. These NO stores regulate NO levels, protecting against excess and supplying it when deficient.

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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Area of Science:

Biochemistry
Physiology
Molecular Biology

Context:

Nitric oxide (NO) is a crucial signaling molecule with a short biological half-life.
Biological systems have evolved mechanisms for NO transport and intracellular storage.
Vascular walls utilize specific complexes for NO homeostasis.

Purpose:

To elucidate the mechanisms of nitric oxide (NO) storage and release.
To investigate the role of S-nitrosothiols and dinitrosyl iron complexes in NO homeostasis.
To explore the potential for therapeutic modulation of NO stores.

Summary:

Nitric oxide (NO) is stored via S-nitrosothiols and dinitrosyl iron complexes, which can interconvert.
These NO stores in the vascular wall buffer against NO overproduction and supply NO during deficiency.
The efficiency of NO storage appears to be genetically determined, linked to an individual's baseline NO production.

Impact:

Understanding NO storage mechanisms can lead to novel therapeutic strategies for NO-related disorders.
Targeting NO store formation and dissociation offers a promising avenue for managing conditions involving NO imbalance.
This research highlights the intricate regulation of NO signaling and its genetic underpinnings.