Evidence of donor suppression of anti-donor PFC responses in mice

Mice receiving high doses of mitomycin C-treated allogeneic spleen donor cells produced more host anti-donor plaque-forming cells than mice receiving high doses of untreated allogeneic spleen donor cells, showing that immunocompetent donor cells capable of recognizing host H-2 antigens can suppress host anti-donor H-2 primary antibody responses. Responses to semi-allogeneic spleen donor cells were consistently lower than responses to allogeneic cells. Low doses of normal allogeneic spleen donor cells showed stimulation of the host anti-donor PFC response compared with similar doses of mitomycin C-treated allogeneic spleen donor cells.