Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of caveolin-1 in human brain microvessels.

D Virgintino1, D Robertson, M Errede

  • 1Department of Human Anatomy and Histology, University of Bari School of Medicine, Piazza Giulio Cesare, I-70124 Bari, Italy. virgintino@histology.uniba.it

Neuroscience
|October 29, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Familial orthostatic tachycardia due to norepinephrine transporter deficiency.

Annals of the New York Academy of Sciences·2001
Same author

The Ca(2+) status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants.

Plant physiology·2001
Same author

Can simultaneous inhibition of seedling growth and stimulation of rhizosphere bacterial populations provide evidence for phytotoxin transfer from plant residues in the bulk soil to the rhizosphere of sensitive species?

Journal of chemical ecology·2001
Same author

Gene pill patents a surprise for gene therapy.

Nature biotechnology·2001
Same author

Origin of the noradrenergic innervation of the superior olivary complex in the rat.

Journal of chemical neuroanatomy·2001
Same author

The Southwestern Ontario Joint Replacement Pilot Project: electronic point-of-care data collection. Southwestern Ontario Study Group.

Canadian journal of surgery. Journal canadien de chirurgie·2001
Same journal

Identification of polyamine metabolism-related gene signatures for prognostic and immunological stratification in glioma.

Neuroscience·2026
Same journal

Age- and sex-dependent effects of single prolonged stress on behavioral, hematological, and oxidative stress parameters in C57BL/6J mice.

Neuroscience·2026
Same journal

Accelerated long-term forgetting and relevant biomarkers for early detection in preclinical Alzheimer's disease.

Neuroscience·2026
Same journal

Deciphering key factors contributing to age-related decline in visuomotor tracking through the manipulation of target refresh rate and gaze contingency.

Neuroscience·2026
Same journal

LPI alleviates Alzheimer's disease pathology via the GPR55 receptor.

Neuroscience·2026
Same journal

Blood-brain barrier dysfunction in surgery and anesthesia: a potential mechanistic contributor to postoperative cognitive dysfunction.

Neuroscience·2026
See all related articles

Caveolin-1 is found in endothelial cells, pericytes, and astrocytes within human cerebral cortex microvessels. This suggests caveolin-1 plays a role in the blood-brain barrier and cell communication.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Caveolae are essential membrane microdomains involved in cellular transport, metabolism, and signal transduction.
  • Caveolins are integral proteins forming the structural framework of caveolae.
  • Different caveolin subtypes are present in the brain, with caveolin-1 and -2 in microvessels and caveolin-3 in astrocytes.

Purpose of the Study:

  • To pinpoint the cellular localization of caveolin-1 within the microvessels of the human cerebral cortex.
  • To investigate the potential role of caveolin-1 in the functioning of the blood-brain barrier.

Main Methods:

  • Immunofluorescence confocal microscopy to visualize caveolin-1 expression in microvessels.
  • Immunogold electron microscopy for high-resolution ultrastructural analysis.

Related Experiment Videos

  • Double immunostaining with glial fibrillary acidic protein to identify astrocytes.

    • Main Results:

    • Caveolin-1 is expressed by endothelial cells and pericytes in all cortical microvessels.
    • Caveolin-1 is also found in astrocytes surrounding the microvessels.
    • Immunoelectron microscopy confirmed caveolin-1's presence in endothelial cells, pericytes, and vascular astrocytes, associated with caveolar structures.

    Conclusions:

    • Caveolin-1 is a component of endothelial cells, pericytes, and astrocytes in human cerebral microvessels.
    • Caveolin-1's presence suggests its involvement in the integrity and function of the blood-brain barrier.
    • The findings support coordinated functions among these cell types within the brain microvasculature.