Related Experiment Video
Updated: Sep 28, 2026

07:03
A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
Published on: September 19, 2025
Effects of nicotine and cotinine on bovine theca interna and granulosa cells
Sara R Sanders1, S Peder Cuneo, Adele M Turzillo
1Department of Physiology, University of Arizona, PO Box 245051, 85724-4347, Tucson, AZ, USA
Reproductive Toxicology (Elmsford, N.Y.)
|October 29, 2002
Abstract:
The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 micro M nicotine decreased (P

