Related Experiment Video
Updated: Aug 19, 2026
Analysis of Cell Migration within a Three-dimensional Collagen Matrix
Published on: October 5, 2014
Hepatocyte growth factor/scatter factor stimulates mitogenesis and migration of a head and neck squamous cell
Jeffery Fleigel1, Jack Sedwick, Lori J Kornberg
1Department of Otolaryngology, University of Florida College of Medicine, Gainesville 32610, USA.
Objective:
We sought to assess the effect of extracellular matrix and hepatocyte growth factor/scatter factor (HGF/SF) on the growth and motility of cultured squamous cell carcinoma of the head and neck (SCCHN) cells.
Methods:
Cultured cells were incubated in the presence of HGF/SF. The effect of HFG/SF on cell growth, motility, and phosphorylation of the signaling proteins FAK and Erk was determined.
Results:
HGF/SF is both mitogenic and motogenic to the human SCCHN cell line FaDu. Incubation of FaDu cells in the presence of HGF/SF led to a rapid increase in phosphorylation of both FAK and the growth-promoting kinase Erk. HGF/SF-induced phosphorylation of FAK and Erk was observed in both detached and attached SCCHN cells. However, phosphorylation was much greater in attached cells.
Conclusions And Significance:
The mitogenic and motogenic activities of HGF/SF may contribute to the pathogenesis of SCCHN in vivo.
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