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Related Experiment Videos

Down-regulation of Delta by proteolytic processing.

Ketu Mishra-Gorur1, Matthew D Rand, Beatriz Perez-Villamil

  • 1Department of Cell Biology, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129, USA.

The Journal of Cell Biology
|October 31, 2002
PubMed
Summary
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Delta cleavage down-regulates Notch signaling by inactivating the Delta ligand. This process is crucial for distinguishing signaling and receiving cells during development.

Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Molecular Biology

Background:

  • Notch signaling controls cell fate decisions via cell-cell interactions.
  • Delta ligand cleavage by Kuzbanian metalloprotease is proposed but functionally debated.

Purpose of the Study:

  • To investigate the functional significance of Delta cleavage in Notch signaling.
  • To determine the biological activity of soluble Delta forms.

Main Methods:

  • In vitro functional assays.
  • In vivo functional assays.
  • Analysis of purified soluble Delta forms.

Main Results:

  • Delta cleavage was confirmed as a significant down-regulating event in Notch signaling.

Related Experiment Videos

  • Purified soluble Delta forms exhibited biological activity.
  • Cleavage inactivates Delta, impacting ligand/receptor expression differentials.

    • Conclusions:

    • Delta cleavage is essential for regulating Notch signaling.
    • This inactivation mechanism distinguishes signaling from receiving cells.
    • A model for Delta inactivation in cell communication is supported.